Issue |
Vet. Res.
Volume 38, Number 6, November-December 2007
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Page(s) | 795 - 808 | |
DOI | https://doi.org/10.1051/vetres:2007032 | |
Published online | 31 August 2007 | |
How to cite this article | Vet. Res. (2007) 795-808 |
DOI: 10.1051/vetres:2007032
Effects of CpG-B ODN on the protein expression profile of swine PBMC
Govindarajulu Nagarajana, Cheng-Chin Kuo*a, Chi-Ming Liangb, c, d, Chi-Min Chene and Shu-Mei Lianga, ba Agricultural Biotechnology Research Center, Academia Sinica, No. 128, Academia Road, Sec. 2, 11529 Taipei, Taiwan
b Genomics Research Center, Academia Sinica, No. 128, Academia Road, Sec. 2, 11529 Taipei, Taiwan
c Institute of Biological Chemistry, Academia Sinica, No. 128, Academia Road, Sec. 2, 11529 Taipei, Taiwan
d Division of Molecular and Genomic Medicine, National Health Research Institutes, Miaoli, Taiwan
e Division of Animal Medicine, Animal Technology Institute Taiwan, Miaoli, Taiwan
(Received 15 September 2006; accepted 26 April 2007; published online 31 August 2007)
Abstract - The CpG motif within bacterial DNA is a potent immuno-stimulatory moiety. Here, using a 2-D electrophoretic approach, we investigated the effect of synthetic oligodeoxynucleotide containing a B type CpG motif (CpG-B ODN) on the protein expression profile of swine peripheral blood mononuclear cells (PBMC). We found that several proteins including spondin 1, N-acetolactate alpha linked acidic dipeptidase; V kappa light chain, T cell receptor variable alpha chain, heat shock protein (Hsp) 60, Hsp70, KIAA0857 protein, and PNAS-146 were up-regulated in PBMC by CpG-B ODN stimulation. Further studies showed that CpG-B ODN-mediated Hsp60, Hsp70 and Hsp90 expressions were closely associated with the TLR9 signalling pathway. Pretreatment with inhibitors of Hsp70, Hsp90 and TLR9 all blocked the CpG-B ODN-mediated anti-apoptotic effect in swine PBMC. These results suggest that CpG-B ODN treatment of swine PBMC may enhance the expression of biologically active proteins, notably spondin 1, V kappa light chain, T cell receptor variable alpha chain and Hsps, which may play an important role in CpG-B ODN-mediated activation of immune responses and enhancement of swine PBMC survival.
Key words: heat shock proteins / CpG ODN / TLR9 / proteomics / apoptosis
Corresponding author: smyang@gate.sinica.edu.tw
© INRA, EDP Sciences 2007