| Issue |
Vet. Res.
Volume 38, Number 6, November-December 2007
|
|
|---|---|---|
| Page(s) | 809 - 818 | |
| DOI | https://doi.org/10.1051/vetres:2007033 | |
| Published online | 31 August 2007 | |
| How to cite this article | Vet. Res. (2007) 809-818 | |
DOI: 10.1051/vetres:2007033
Bacterial lipopolysaccharide stimulates bovine neutrophil production
of TNF-
, IL-1
, IL-12 and IFN-
Eun J. Sohna, Max J. Paapeb, Erin E. Connorb, Douglas D. Bannermanb, Raymond H. Fettererc and Robert R. Petersa a Department of Animal and Avian Sciences, University of Maryland, College Park, MD 20742, USA
b Bovine Functional Genomics Laboratory, USDA-ARS, Beltsville, MD 20705, USA
c Animal Parasitic Diseases Laboratory, USDA-ARS, Beltsville, MD 20705, USA
(Received 8 November 2006; accepted 14 May 2007; published online 31 August 2007)
Abstract - After intramammary infection, polymorphonuclear
neutrophil leukocytes (PMN) are the first cells recruited into the mammary
gland. Rapid recruitment of and bacterial phagocytosis and killing by PMN
are the most effective defenses against establishment of bacterial
infection. In addition to their phagocytic and bactericidal properties, PMN
may play a key supportive role through secretion of cytokines during the
innate immune response. We sought to determine whether bovine PMN produce
cytokines in response to stimulation by lipopolysaccharide (LPS). To
investigate the effects of LPS on the expression of cytokines secreted by
bovine PMN, we measured the expression of tumor necrosis factor
(TNF)-, interleukin (IL)-1, IL-12, and interferon
(IFN)- by ELISA after stimulation with different concentrations of
LPS, and secretion of IL-8 after co-stimulation with LPS and either
TNF- or IL-1. Bovine PMN were shown to secrete TNF-, IL-1, IL-12, IL-8 and IFN- in response to LPS.
Co-incubation of PMN with LPS and TNF- increased secretion of IL-8
when compared to LPS alone. It was concluded that LPS stimulation
up-regulates the secretion of cytokines by bovine PMN, and that
co-incubation of LPS with TNF- had an additive effect on the
secretion of IL-8. These data show that bovine PMN, in addition to their
phagocytic and bactericidal properties, may play a supportive role in the
innate immune response to infection by Gram-negative bacteria through their
ability to produce immuno-regulating cytokines.
Key words: lipopolysaccharide / bovine / neutrophils / cytokines / mastitis
Corresponding author: mpaape@anri.barc.usda.gov
© INRA, EDP Sciences 2007