Estimated numbers of MAIT cells within the T cell populations in tissues of cattle and sheep. Samples of cDNA prepared from bovine and ovine tissues were analysed by qPCR to determine the number of MAIT cell TCRα chain transcripts and the number of α chain constant segment transcripts present. Numbers of transcripts were calculated by comparison with a standard curve that was generated using a cloned full-length MAIT cell α chain. Results are presented as quantities of MAIT TCRα chain expressed as a percentage of total α chain transcripts. Each experiment was performed three times on the same tissues from each animal. Per LN: peripheral lymph node; Mes LN: mesenteric lymph node; SI wall: small intestinal wall; LI wall: large intestinal wall.