Effect of blockade of the αvβ3 integrin on bovine neutrophil chemotaxis. Compared to the control, neutrophil migration was significantly higher in fMLP alone or with isotype-matched antibody or the αvβ3 integrin antibody but was not different among the three fMLP treatments. While RGDSK/K–RNT alone significantly reduced the fMLP-induced migration, the effect was not noticed in neutrophils pretreated with the αvβ3 integrin mAb. Modified RPMI-1640 and fMLP (114 nM) in the lower chamber were used as negative and positive controls, respectively. Results of three independent experiments are displayed as mean ± SEM. Significant differences between treatment groups are expressed by different letters above bars (p < 0.001).