Open Access

Figure 4.


Inhibition of bovine neutrophil chemotaxis induced by RGDSK/K–RNT or MAPK inhibitors. Neutrophil migration, determined by counting the number of neutrophils stuck in filter pores after 30 min of chemotaxis assay, was significantly diminished after exposure to RGDSK/K–RNT for 5 min or MAPK inhibitors for 1 h. Modified RPMI-1640 and fMLP (114 nM) in the lower chamber were used as negative and positive controls, respectively. DMSO (dimethyl sulfoxide), a solvent of MAPK inhibitors, was used as a negative control. Results of three independent experiments are displayed as mean ± SEM. Significant differences between treatment groups are expressed by different letters above bars (p < 0.001).

Download original image