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Table I.
Flow cytometric analysis of T cell marker expression in equine PBMC (n = 4) after stimulation with PMA and ionomycin (PMA) or in non-stimulated cells (med).
| Cell population (%)a | Time of stimulation (hours) |
|||||
|---|---|---|---|---|---|---|
| 2 | 4 | 6 | 8 | 18 | 24 | |
| CD3+ (med)b | 77.6 ± 9.4 | 79.3 ± 8.3 | 81.7 ± 7.4 | 79.9 ± 7.4 | 86.4 ± 5.9 | 86.3 ± 3.1 |
| CD3+ (PMA)b | 84.4 ± 3.6 | 83.5 ± 6.2 | 85.1 ± 6.3 | 86.9 ± 4.0 | 87.5 ± 3.6 | 87.5 ± 3.2 |
| Ratio CD3+ med/PMA | 0.92 | 0.95 | 0.96 | 0.92 | 0.99 | 0.99 |
| CD4+ (med)c | 76.3 ± 1.3 | 75.7 ± 1.6 | 76.5 ± 1.1 | 76.7 ± 1.2 | 77.1 ± 2.2 | 75.8 ± 2.2 |
| CD4+ (PMA)c | 55.7 ± 3.5 | 31.4 ± 5.1 | 37.8 ± 6.0 | 26.8 ± 8.7 | 14.0 ± 10.4 | 8.3 ± 3.5 |
| Ratio CD4+ med/PMA | 1.37 | 2.41 | 2.03 | 2.86 | 5.50 | 9.11 |
| CD8+ (med)c | 17.1 ± 1.2 | 18.2 ± 1.8 | 17.4 ± 1.2 | 17.2 ± 1.8 | 16.2 ± 1.3 | 15.4 ± 1.1 |
| CD8+ (PMA)c | 10.7 ± 2.0 | 8.8 ± 1.1 | 11.0 ± 1.5 | 11.5 ± 1.4 | 9.0 ± 1.2 | 8.9 ± 1.8 |
| Ratio CD8+ med/PMA | 1.60 | 2.06 | 1.58 | 1.49 | 1.8 | 1.73 |