Effect of strain and inoculation dose of classical swine fever virus on within-pen transmission | Veterinary Research, a journal on Animal Infection

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Table I.

Clinical symptoms, blood parameters, serology, virus isolation and RRT-PCR results.

Virus strain and dose^a	CS^b	Fever^c	Leukopenia^d	VI leukocytes^e	ELISA^f	NPLA titre^g	RRT-PCR on tonsils
Zoelen – 10⁵ TCID₅₀
I	no	no	yes	5–7	+	640	+
I	no	no	no	5–7	+	320	+
I	no	no	yes	5–7	+	320	+
Paderborn middle – 10^3.5 TCID₅₀
I	yes	no	yes	7–12	+	80	+
I	yes	yes	yes	5–23^†	+	20	+
I	yes	yes	yes	5–35	+	< 5	+
C	yes	yes	yes	17–35	+	< 5	+
C	yes	yes	yes	14–35	+	5	+
C	yes	yes	yes	12–35	+	< 5	+
C	yes	yes	yes	17–29^†	+	20	+
C	yes	yes	yes	14–35	+	< 5	+
C	yes	yes	yes	14–35	+	< 5	+
C	yes	yes	yes	17–28	+	80	+
Paderborn high – 10⁵ TCID₅₀
I	yes	yes	yes	5–32^†	−	< 5	+
I	yes	yes	yes	3–21	+	20	+
I	yes	yes	yes	3–21^†	−	< 5	+
C	yes	yes	yes	17–35	+	10	+
C	yes	yes	yes	17–35	+	< 5	+
C	yes	yes	yes	17–35	+	< 5	+
C	yes	yes	yes	17–24	+	80	+
C	yes	yes	yes	14–21	+	20	+
C	yes	yes	yes	10–35	−	< 5	+
C	yes	yes	yes	14–31^†	+	5	+
Brescia – 10^2.5 TCID₅₀
I	yes	yes	yes	3–14^†	+	< 5	+
I	yes	yes	yes	3–13^†	−	< 5	+
I	yes	yes	yes	3–11^†	−	< 5	+
C	yes	yes	yes	10–21^†	+	< 5	+
C	yes	yes	no	10–13^†	−	< 5	+
C	yes	yes	yes	10–14^†	−	< 5	+
C	yes	yes	yes	10–21^†	+	< 5	+
C	yes	yes	yes	10–18^†	+	< 5	+
C	yes	yes	yes	10–13^†	−	< 5	+
C	yes	yes	yes	10–12^†	−	< 5	+

^†

Day of death or euthanasia (p.i.).

Contact pigs of the Zoelen trial and pigs from the Paderborn low inoculation dose trial were not included in the table since none of the tests showed a positive result.

Clinical symptoms: CS > 2.

Body temperature for 2 or more consecutive days > 40 °C.

Leukopenia: < 10 × 10⁹ leukocytes/L blood.

Interval in days p.i. when virus isolation on leukocytes were positive.

ELISA on serum samples: + positive, − negative.

NPLA-titre in serum samples.