Open Access

Table I.

Oligonucleotide primers.

Primer Sequence (5’–3’)a
Afadé_EcoRI GATCgaattcATGAAGCAAACAAAAGTTGATATTTG
MmmSC_GlpO_BamHI ATATggatccTTTCCATGGAAGAATAGCTTC
MmmSC_glpO_FAD1F GATATTTGTATCATTGGAATTATTGGTGCTTCTGTTGCAAGAGAA
MmmSC_glpO_FAD1R AGCACCAATAATTCCAATGATACAAATATCAACTTTTGTTTGCTT
MmmSC_glpO_FAD2F GATATTTGTATCATTATTGGTGCTTCTGTTGCAAGAGAACTAGCA
MmmSC_glpO_FAD2R AACAGAAGCACCAATAATGATACAAATATCAACTTTTGTTTGCTT
MmmSC_glpO_FAD3F GATATTTGTATCATTGCTTCTGTTGCAAGAGAACTAGCAAAATTT
MmmSC_glpO_FAD3R TCTTGCAACAGAAGCAATGATACAAATATCAACTTTTGTTTGCTT
pETHIS-1_EcoRI GATCgaattcTCTCCTTCTTAAAGTTAAACAAAA
pETHIS-1_BamHI ATATggatccCACCATCACCATCACCATC
a

Lower-case letters indicate nucleotides added to create restriction enzyme recognition sites for cloning.