Issue |
Vet. Res.
Volume 37, Number 6, November-December 2006
|
|
---|---|---|
Page(s) | 779 - 790 | |
DOI | https://doi.org/10.1051/vetres:2006037 | |
Published online | 15 September 2006 | |
How to cite this article | Vet. Res. (2006) 779-790 |
DOI: 10.1051/vetres:2006037
Mycoplasma mycoides subsp. capri and Mycoplasma mycoides subsp. mycoides LC can be grouped into a single subspecies
Edy M. Vilei, Bozena M. Korczak and Joachim FreyInstitute of Veterinary Bacteriology, Universität Bern, Länggass-Strasse 122, Postfach, 3001 Bern, Switzerland
(Received 6 February 2006; accepted 10 May 2006; published online 15 September 2006)
Abstract - Mycoplasma mycoides subsp. capri and Mycoplasma mycoides subsp.
mycoides LC can be combined into one taxon on the basis of several
contributions on both DNA sequence and protein analyses reported in the
literature. Moreover, for the differentiation and identification of
mycoplasmas of the "mycoides cluster", we investigated the rpoB gene,
encoding the -subunit of the RNA polymerase. A segment of 527 bp of
the rpoB gene was amplified from 31 strains of ruminant mycoplasmas by PCR.
The nucleotide sequences were determined and aligned, and accurate genetic
relationships were calculated. Cluster analysis of rpoB DNA allowed species
differentiation within the "mycoides cluster" and confirmed that M. mycoides
subsp. capri and M. mycoides subsp. mycoides LC cannot be distinguished from
each other. "Mycoplasma mycoides subsp. capri" is proposed as a common
name for both subspecies
Key words: Mycoplasma mycoides subsp. capri / Mycoplasma mycoides subsp. mycoides LC / ruminant mycoplasmoses / taxon / serovar
Corresponding author: edy.vilei@vbi.unibe.ch
© INRA, EDP Sciences 2006