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Figure 3.


Transcription of IL-10, TGF-β and Foxp3 in CD4CD25 and γδ T cell sorted bovine cell subpopulations as determined by qRT-PCR. (a) CD4+CD25high, CD4+CD25low, CD4+CD25-, and CD4-CD25- cells were FACS sorted (CD4FITC/ CD25PE) from cow A–C and (b) WC1+, WC1.2+ and WC1.1+ PE stained γδ T cells were sorted from cow A and C. Results show mean fold changes in gene expression (±1SEM) of triplicate samples from three representative experiments of specific cell populations compared to total PBMC, calculations based on REST© (Relative Expression Software Tool) software [29] relative to the gene expression of β2-microglobulin.

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