Transcription of IL-10, TGF-β and Foxp3 in CD4CD25 and γδ T cell sorted bovine cell subpopulations as determined by qRT-PCR. (a) CD4⁺CD25^high, CD4⁺CD25^low, CD4⁺CD25^-, and CD4^-CD25^- cells were FACS sorted (CD4FITC/ CD25PE) from cow A–C and (b) WC1⁺, WC1.2⁺ and WC1.1⁺ PE stained γδ T cells were sorted from cow A and C. Results show mean fold changes in gene expression (±1SEM) of triplicate samples from three representative experiments of specific cell populations compared to total PBMC, calculations based on REST^© (Relative Expression Software Tool) software [29] relative to the gene expression of β2-microglobulin.