Transcription of IL-10, TGF-β and Foxp3 in 7 sorted bovine cell subpopulations as determined by qRT-PCR. (a) PBMC were isolated from cows A-C, respectively PE stained and sorted CD3⁺, CD4⁺, CD8⁺, N24⁺, CD21⁺, CD14⁺, NKp46⁺ cells were tested. Results show mean fold changes in gene expression (±1SEM) of triplicate samples from three representative experiments of specific cell populations compared to total PBMC, calculations based on REST^© (Relative Expression Software Tool) software [29] relative to the gene expression of β2-microglobulin. (b) MW sizes of products of qRT-PCR (6 independent PCR), for β2-microglobulin (171 bp), Foxp3 (88 bp), IL-10 (112 bp) and TGF-β (141 bp) (cow A) performed on bovine PBMC derived samples on a 2% agarose gel and compared to a MassRuler™ low range DNA ladder.