CD4CD25, WC1.2⁺ and WC1.1⁺expression on stained bovine PBMC. (a) Representative dotplot of CD4FITC/CD25PE double-color surface stained PBMC (cow A) and gate regions for sorting and/or frequency analysis of CD4⁺CD25^- (gate R1), total CD25⁺ (gate R2), CD4⁺CD25^low (gate R3), CD4⁺CD25^high (gate R4) and CD4^-CD25^- (gate R5) cells. (b) Representative dotplots of WC1.2⁺, (gate R6) respectively (c) WC1.1⁺ (gate R7) γδ T cell subsets stained PBMC and gate regions for sorting and frequency analysis.