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Figure 1.


CD4CD25, WC1.2+ and WC1.1+expression on stained bovine PBMC. (a) Representative dotplot of CD4FITC/CD25PE double-color surface stained PBMC (cow A) and gate regions for sorting and/or frequency analysis of CD4+CD25- (gate R1), total CD25+ (gate R2), CD4+CD25low (gate R3), CD4+CD25high (gate R4) and CD4-CD25- (gate R5) cells. (b) Representative dotplots of WC1.2+, (gate R6) respectively (c) WC1.1+ (gate R7) γδ T cell subsets stained PBMC and gate regions for sorting and frequency analysis.

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