Free Access
Vet. Res.
Volume 40, Number 6, November-December 2009
Number of page(s) 10
Published online 27 June 2009
How to cite this article Vet. Res. (2009) 40:55
How to cite this article: Vet. Res. (2009) 40:55
DOI: 10.1051/vetres/2009038

Identification of a multidrug efflux pump in Flavobacterium johnsoniae

Sarah E. Clark1, 2, Brooke A. Jude1, 3, G. Russell Danner1, 4 and Frank A. Fekete1

1  Department of Biology, Colby College, 5729 Mayflower Hill Drive, Waterville, ME 04901, USA
2  Present address: Department of Cell & Molecular Biology, Graduate Program in Microbiology, Virology, and Parasitology, University of Pennsylvania, Philadelphia, PA 19104, USA
3  Present address: Department of Biology, Bard College, Annandale-on-Hudson, NY 12504, USA
4  Maine Department of Inland Fisheries & Wildlife, Augusta, ME 04330, USA

Received 27 February 2009; accepted 25 June 2009; published online 27 June 2009

Abstract - In this study, the mechanism conferring multiple drug resistance in several strains of flavobacteria isolated from the ovarian fluids of hatchery reared 3-year old brook trout Salvelinus fontinalis was investigated. Metabolic fingerprinting and 16S rRNA gene sequences identified the isolates as Flavobacterium johnsoniae. The isolates exhibited multiple resistances to a wide range of antimicrobial classes including penicillin, cephem, monobactam, aminoglycoside, and phenicol. Although plasmids and other transposable elements containing antimicrobial resistance genes were not detected, the isolates did contain a genomic sequence for a chloramphenicol-inducible resistance-nodulation-division family multidrug efflux pump system. Efflux pumps are non-specific multidrug efflux systems. They are also a component of cell-cell communication systems, and respond specifically to cell membrane stressors such as oxidative or nitrosative stress. Understanding of efflux pump mediated antibiotic resistances will affect efficacy of clinical treatments of fishes associated with F. johnsoniae epizootics.

Key words: Flavobacterium johnsoniae / multidrug efflux pump / antibiotic resistance / aquaculture / brook trout

Corresponding author:

© INRA, EDP Sciences 2009