Open Access
Vet. Res.
Volume 40, Number 4, July-August 2009
Number of page(s) 14
Published online 24 April 2009
How to cite this article Vet. Res. (2009) 40:40
How to cite this article: Vet. Res. (2009) 40:40
DOI: 10.1051/vetres/2009023

The food contaminant fumonisin B1 reduces the maturation of porcine CD11R1+ intestinal antigen presenting cells and antigen-specific immune responses, leading to a prolonged intestinal ETEC infection

Bert Devriendt1, Me'lanie Gallois2, Frank Verdonck1, Yann Wache2, Diane Bimczok3, 4, Isabelle P. Oswald2, Bruno M. Goddeeris1, 5 and Eric Cox1

1  Laboratory of Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium
2  Laboratoire de Pharmacologie-Toxicologie, UR 66, Institut National de la Recherche Agronomique, Toulouse, France
3  Institute of Anatomy, Otto-von-Guericke University, Leipziger Strasse 44, 39120 Magdeburg, Germany
4  Present affiliation: Department of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA
5  Vaccine design, Department of Biosystems, K.U. Leuven, Kasteelpark Arenberg 30, B-3001 Heverlee, Belgium

Received 10 December 2008; accepted 23 April 2009; published online 24 April 2009

Abstract - Consumption of food or feed contaminated with fumonisin B1 (FB1), a mycotoxin produced by Fusarium verticillioides, can lead to disease in humans and animals. The present study was conducted to examine the effect of FB1 intake on the intestinal immune system. Piglets were used as a target and as a model species for humans since their gastro-intestinal tract is very similar. The animals were orally exposed to a low dose of FB1 (1 mg/kg body weight FB1) for 10 days which did not result in clinical signs. However, when compared to non-exposed animals, FB1-exposed animals showed a longer shedding of F4+ enterotoxigenic Escherichia coli (ETEC) following infection and a lower induction of the antigen-specific immune response following oral immunization. Further analyses to elucidate the mechanisms behind these observations revealed a reduced intestinal expression of IL-12p40, an impaired function of intestinal antigen presenting cells (APC), with decreased upregulation of Major Histocompatibility Complex Class II molecule (MHC-II) and reduced T cell stimulatory capacity upon stimulation. Taken together, these results indicate an FB1-mediated reduction of in vivo APC maturation.

Key words: fumonisin B1 / intestinal APC / F4(K88)+ enterotoxigenic Escherichia coli / mucosal immunization

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© INRA, EDP Sciences 2009