Issue |
Vet. Res.
Volume 39, Number 1, January-February 2008
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Number of page(s) | 12 | |
DOI | https://doi.org/10.1051/vetres:2007043 | |
Published online | 09 November 2007 | |
How to cite this article | Vet. Res. (2008) 39:06 |
DOI: 10.1051/vetres:2007043
Bovine IgG1 antibodies against Mycobacterium avium subsp. paratuberculosis protein p34-cx improve association of bacteria and macrophages
Silvia L. Mundo1, Adriana M. Fontanals1, Mariana García2, María Durrieu1, Elida Álvarez2, Elida R. Gentilini1 and Silvia E. Hajos21 Cátedra de Inmunología, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires (UBA), Argentina
2 Cátedra de Inmunología, Facultad de Farmacia y Bioquímica, IDEHU, UBA-CONICET, Argentina
(Received 17 October 2006; accepted 6 August 2007; published online 9 November 2007)
Abstract - Paratuberculosis, caused by Mycobacterium avium subsp.
paratuberculosis (Map), is a chronic granulomatous enteric disease in
cattle. Among molecular components of Map, protein p34 was identified as
specific and immunodominant for bovine B cells. In order to determine if
specific antibodies could influence the course of Map pathogenesis, the
interaction between bacteria and bovine macrophages was studied. Bovine
polyclonal antibodies from 3 calves vaccinated with protein p34-cx, 6 calves
vaccinated with heat-killed Map, 8 naturally infected, and 3 healthy calves
-as negative controls- were used. Specific anti-Map, -p34-cx and -PPA-3
antibodies were evaluated and isotype characterized. Infected and Map
vaccinated animals showed similar IgG1 and IgG2 response against Map whole
bacteria. When p34-cx was used as the antigen, mainly IgG1 and IgG3 were
detected in infected and only IgG1 in p34-cx vaccinated animals. Bovine
polyclonal antibodies from three animals of each category were isolated and
affinity purified through Map and p34-cx columns. The effect of these
antibodies in association with Map and a transformed bovine peritoneal
macrophage's cell line (Bov-Mac) as well as activation of NF-B
transcription factor was studied. Our results show that association of Map
significantly increased in vitro after pretreatment with bovine anti-Map or
anti-p34-cx antibodies obtained from vaccinated or infected cattle when
compared with those of controls. Improved activation of NF-
B was
detected in macrophages that ingested Map opsonized with either anti-Map or
anti-p34-cx specific antibodies of infected or vaccinated calves, suggesting
that both anti-Map and IgG1 anti-p34-cx antibodies support Map-macrophage
interactions.
Key words: Mycobacterium avium subsp. paratuberculosis / p34 / IgG1 / macrophages / cattle
Corresponding author: smundo@fvet.uba.ar
© INRA, EDP Sciences 2007