Issue |
Vet. Res.
Volume 34, Number 2, March-April 2003
|
|
---|---|---|
Page(s) | 177 - 184 | |
DOI | https://doi.org/10.1051/vetres:2002064 | |
How to cite this article | Vet. Res. (2003) 177-184 |
DOI: 10.1051/vetres:2002064
An anti-human recombinant tumor necrosis factor
alpha (TNF
) monoclonal antibody recognizes
an epitope in feline TNF
Adam Aguirre, Alejandro Escobar, Viviana Ferreira, María C. Molina, Arturo Ferreira and Juan C. Aguillón Disciplinary Program of Immunology, Institute of Biomedical Sciences (ICBM), Faculty of Medicine, University of Chile, Independencia 1027, Casilla 13898, Correo 21, Santiago, CHILE
(Received 11 February 2002; accepted 10 October 2002)
Abstract
It is likely that the murine response to human recombinant TNF
(hrTNF
)
may generate antibodies (Ab) to epitopes present in TNF
from other species.
Here, we demonstrate that F5 anti-hrTNF
monoclonal antibody (mAb) recognizes
feline TNFa while E8 anti-hrTNF
mAb failed to do so. In order to demonstrate
that E8 and F5 mAb recognize different epitopes in the hrTNF
molecule, a constant
concentration of E8 and variable concentrations of F5 were incubated with solid phase
bound hrTNF
. Binding of E8 and F5 to hrTNF
was determined with
anti-
and
chain specific Ab. F5 bound equally to hrTNF
in the presence
or absence of E8 and the same amount of E8 bound to hrTNF
, in spite of the presence
of F5. When using the E8 and F5 mAb for capturing the TNF
from the equine, canine,
feline and bovine species, in supernatants of an ex vivo lipopolysaccharide (LPS)-stimulated
whole blood cell culture, we only detected the feline TNF
by F5 mAb (
p = 0.001).
By a cytotoxic assay on L929 fibroblasts, we indeed demonstrated the feline TNF
production after the LPS stimulus. In an inhibition assay, the human and feline
cytokines competed for F5, although the inhibition of native human TNF
binding
to F5 was significant but only about 20% (
p = 0.001). In conclusion, most likely
the F5 anti-hrTNF
mAb recognizes an epitope in feline TNF
.
Its immunomodulatory potential in the feline model remains to be studied.
Key words: Monoclonal antibodies / Tumor necrosis factor alpha / Cross-reactivity
Correspondence and reprints: Juan C. Aguillón Tel.: (56) 2 678.6724; fax: (56) 2 735.3346;
e-mail: jaguillo@machi.med.uchile.cl
© INRA, EDP Sciences 2003