Porcine monocyte subsets differ in the expression of CCR2 and in their responsiveness to CCL2 | Veterinary Research, a journal on Animal Infection

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Table I.

Primers and PCR conditions used in this study.

Molecule	Primers	Sequence	Product	T_an
CCR7	CCR7F	GGC TCA AGA CCA TGA CCG AC	712
	CCR7R	CCG ATG AAG GCG TAC AAG
CCR4	CCR4F	CTC CCT GAG AGC AAG GAC	364
	CCR4R	CTC CAG GAA GAG CAC CAC
CXCR3	CXCR3F	GCC CTC TTC AAC ATC AAC TTC	612	52
	CXCR3R	CAG CAT CCA CAT CCG CTC
CX₃CR1	CX3CR1F	CAA CAG CAA GAA GCC CAA G	444
	CX3CR1R	AGC AGG GGG AGT AGG AAG
CCR2	CCR2F	TTG TGT GAC CCA AGA GAG ACT TAC G	529	58
	CCR2R	GTT ACA GCC AAA CCA TCC TAA AGC
CCR5	CCR5F	GCA ATG AGA AGA AGA AGC ACA AGG	493	55
	CCR5R	GGA GCA GAT GCC AAA CGG ATA G
CXCR4	CXCR4F	TTA CAC AGA GGA TGA CTT GGG CTC	707	57
	CXCR4R	GGA TGA GGA TAA CGG TGG TTT TG
CCR1	CCR1F	AAGGGCTATGATGCAACCAC	504	53
	CCR1R	CTGGGTCTTGGAAAAGTGGA
CCR6	CCR6F	CTGGTGACGGCCGTCAACCTG	191	60
	CCR6R	TTCACACACCACAGGTCTTTCATGATC
CCL2	CCL2F	CCTCATCCTCCAGCATGAAGGTCTCTGC	328	62
	CCL2R	GGTGGAGTCAGGCTTCAAGGCTTCGG
CCL2-GFP	CCL2GFPR	GAGGCTTCGGAGTTTGGTTTTTCTTGTCCAGG	313	62
CCL2-V5	CCL2V5R	AGGCTTCGGAGTTTGGTTTTTCTTGTCCAGG	312	62
GAPDH	GAPDHF	CCATCACCATCTTCCAGGAGCGAG	285	55
	GAPDHR	AAGTTGTCATGGATGACCTTGGCCA
B-Actin	B-Actin-F	TGCTGTCCCTGTACGCCTCTG	220	55
	B-Actin-R	ATGTCCCGCACGATCTCCC