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Figure 1.

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Cellular localisation of sortase A (SrtA) in Streptococcus uberis. Protein extracts were obtained from S. uberis 0140J and srtA mutant from cultures grown overnight in THB media. Fractions were separated on 15% SDS PAGE gels, transferred to nitrocellulose and probed with antiserum to SrtA (size of proteins of known molecular mass are indicated). Extracts were loaded as follows: from S. uberis 0140J capsule (lane 1); mutanolysin-derived cell wall (lane 3); cytoplasmic (lane 5); cell membrane (lane 7) and precipitated media extracts (lane 9) and from the srtA mutant capsule (lane 2); mutanolysin-derived cell wall (lane 4); cytoplasmic (lane 6); cell membrane (lane 8) and precipitated media extracts (lane 10). (A color version of this figure is available online at www.vetres.org.)

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