The mouse model is suitable for the study of viral factors governing transmission and pathogenesis of highly pathogenic avian influenza (HPAI) viruses in mammals | Veterinary Research, a journal on Animal Infection

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Table V.

Quantitative Real-Time PCR and viral isolation results of H5N1 contact mice (G2).

ID number	Detail	Organs^b	qRRT-PCR	Copies/μL	Virus isolation
(A) G2_Tk/05 contact mice
1	Dead (10th p.c)	Brain	+	5.5E + 09	+
		Lung/trachea	+	3.05E + 06	+
		Spleen	+	4.3E + 04	−
		Intestinal content	+	2.7E + 05	−
2	Dead (10th p.c.)	Brain	+	1.9E + 06	+
2	Dead (10th p.c.)	Lung/trachea	+	2.7E + 05	+
3	Dead (12th p.c.)	Brain	NA^a	NA	NA
3	Dead (12th p.c.)	Lung/trachea	+	2.2E + 05	−
4	Dead (12th p.c.)	Brain	+	3.3E + 08	+
		Lung/trachea	+	6.4E + 05	+
		Spleen	+	2.2E + 05	−
5	Sacrificed^* (20th p.c.)	Brain	+	3.05E + 04	−
5	Sacrificed^* (20th p.c.)	Spleen	+	5.96E + 04	−
6	Sacrificed^* (20th p.c.)	Brain	+	4.82E + 05	+
(B) G2_Mal/835 contact mice
1	Sacrificed^**(10th p.c.)	Lung/trachea	+	2.68E + 05	+

Mice sacrificed for ethical reason (severe signs of disease).

^**

Animal sacrificed according to the experimental protocol.

Not analyzed. Samples found putrefied could not be processed.

From G2 mice brain, lung, spleen, liver, kidney and intestinal content were collected.

This table shows the number of mice and the type of tissues found positive when first processed by qRRT-PCR.