Open Access

Table II.

VM and scrapie detection in the lung and mammary gland in Sc+/VM+ infected sheep.

Sc+/VM+ with VMV-induced lymphoid follicle hyperplasia (LFH)
Sc+/VM+ without VMV-induced LFH
Sheep1
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
Organ2 L M L M L M L M L M L M L M L M L M L M L M L M L M L M L M L M L M
VM Lesion3 ++ + + + + + + + + ++ + + + + + + + + + + +
PCR4 + + + + + + + + + + + + + + + + + + + + +
Sc IHC5 + + + + + + + + + + +
WB6 + + + + + +
BioRad7 + + + + +
IDEXX8 + + + + + + +
Final Result9 + + + + + + + + + + + + +
Ln10 + + + + + + + + + + + + + + + + + + + + + +
1

All animals were positive for detection of VMV antibodies (ELITEST, Hyphen Biomed).

2

Organ: L = lung, M = mammary gland.

3

Lesion: presence of VMV-related LFH.

4

PCR: detection VMV-LTR sequences.

5

IHC: detection of PrPSc by immunohistochemistry.

6

WB: detection of PrPSc by Western blotting (Prionics®-Check WESTERN SR, Prionics AG).

7

BioRad: detection of PrPSc by BioRad ELISA (BioRad TeSeE sheep/goat).

8

IDEXX: detection of PrPSc by IDEXX ELISA (IDEXX HerdCheck).

9

Final Result: negative (−): all techniques for PrPSc detection were negative; Positive (+): IHC and/or WB were positive.

10

Ln: IHC for PrPSc in the associated lymph node (l: mediastinal lymph node, m: mammary lymph node).