Phenotypic maturation of MoDC, FL-DC and GM-DC upon stimulation with different TLR ligands in terms of up regulation of MHC II and CD86. The different DC cultures were stimulated for 24 h with Pam₃Cys-SK₄ (Pam3C, 10 μg/mL), polyinosinic-polycytidylic acid potassium salt (PIC, 10 μg/mL), LPS (1 μg/mL), Imiquimod (Imi, 5 μg/mL) or CpG 2395 (5 μg/mL). The ratios of the MFI of stimulated and unstimulated cells were calculated and tested for significance (n = 5; *p value of 0.01–0.05; **p > 0.01). (A color version of this figure is available online at www.vetres.org.)