Primary bovine spleen cells, MNC and BL3 cells respond to both, di- and triacylated LP. (A) Bovine spleen cells were stimulated for 24 h with 1, 10 and 100 nM of di- and triacylated LP with the peptide moieties FEPPPATTT, SK₄ or GDPKHPKSF. Activation was measured by [³H]TdR incorporation into the B-lymphocytes and results are expressed in counts per minute per culture. Values are means ± SD of n = 3 and represent the result of 3 independent experiments. (B) Primary bovine MNC and (C) BL3 cells were stimulated for 24 h with 100 nM of Pam₂C-SK₄ or Pam₃C-SK₄. IL-8 induction was determined on mRNA level by real-time PCR normalized to the house-keeping gene HPRT. The results are representative for at least 3 independent experiments.