Cotransfection of boTLR2 with muTLR1 but not with bovine or human TLR1 resulted in the recognition of triacylated LP. HEK293 cells were transfected with (A) boTLR2 alone or in combination with (B) boTLR1-s, (C) huTLR1 or (D) muTLR1. Cells were stimulated for 24 h with 100 nM of Pam₂C-SK₄ or Pam₃C-SK₄. IL-8 production was measured in the supernatant. Values are means ± SD of n = 2 and represent the result of at least 3 independent experiments.