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Table II.

Serological analysis of sera from swine inoculated with rS. cho.

Group Animal no. Neutralizing antibodya
Mean % inhibition of antibody against 3ABCb ± SD
Day 0 Day 7 Day 14 Day 0 Day 7 Day 14
Saline 28-8 ≤ 3 108 362 NDc ND ND
1-48 ≤ 3 362 362 ND ND ND
3-2 ≤ 3 128 362 2.6 ± 0.2 39.5 ± 0.1 36.2 ± 2.0
S. cho 6-4 ≤ 3 362 362 12.9 ± 2.9 42.9 ± 1.1 55.9 ± 1.1
4-6 ≤ 3 180 362 13.6 ± 1.9 54.5 ± 1.9 63.2 ± 0.7
pLacZ/S. cho 6-2 ≤ 3 180 362 ND ND ND
43-14 ≤ 3 180 362 ND ND ND
43-5 ≤ 3 256 256 ND ND ND
49-3 ≤ 3 128 180 ND ND ND
p3D-NT56/S. cho 2-7 ≤ 3 23 180 12.16 ± 1.1 12.6 ± 2.4 25.8 ± 0.4
5-6 ≤ 3 12 180 6.5 ± 1.0 10.1 ± 0.3 27.8 ± 4.2
3-3 ≤ 3 12 180 12.8 ± 0.8 9.5 ± 0.2 32.9 ± 5.2
1-1 ≤ 3 23 180 11.9 ± 0.5 21.2 ± 3.8 29.5 ± 2.7
p3D-NT56/S. cho d 1-3 ≤ 3 108 128 3.1 ± 1.0 36.5 ± 1.2 50.0 ± 4.3
7-1 ≤ 3 23 180 6.4 ± 0.0 27.9 ± 0.2 33.6 ± 3.2
3-4 ≤ 3 108 180 7.4 ± 0.7 39.1 ± 1.8 45.5 ± 0.2
1-34 ≤ 3 64 180 ND ND ND
a

Highest dilution that resulted in a 50% reduction in the number of HKN/2002 plaques.

b

Mean percent inhibition of the optical density as determined by ELISA.

c

ND: not determined.

d

Inoculation with a high dose of rS. cho.