Apoptosis induction in BEFV-infected Vero and MDBK cells through Src-dependent JNK activation regulates caspase-3 and mitochondria pathways | Veterinary Research, a journal on Animal Infection

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Table I.

SP600125 and SU6656 alleviated BEFV-mediated apoptosis.

	Vero			MDBK
	Flow cytometry (% subG1)	Apoptotic ELISA (enrichment factor)	MTT (% viability)	Flow cytometry (% subG1)	Apoptotic ELISA (enrichment factor)	MTT (% viability)
Mock	2.2 ± 0.15	1	100	2.8 ± 0.6	1	100
BEFV 2 moi	67.5 ± 6.1	8.92 ± 1.42	28.6 ± 1.6	23.3 ± 2.4	9.05 ± 1.13	40.6 ± 3.5
BEFV+PD98059	60.2 ± 5.7	7.45 ± 2.12	25.4 ± 2.6	21.1 ± 2.1	7.21 ± 1.24	41.6 ± 2.9
BEFV+SB203580	63.3 ± 5.3	8.08 ± 1.63	31.2 ± 2.7	19.9 ± 1.7	7.68 ± 1.41	39.1 ± 3.1
BEFV+SP600125	23.5 ± 3.5	2.85 ± 0.91^*	76.2 ± 4.6^*	8.6 ± 1.9	2.35 ± 0.82^*	80.5 ± 3.7^*
BEFV+LY294002	59.9 ± 5.4	8.56 ± 1.93	32.2 ± 2.2	24.3 ± 2.3	7.07 ± 0.93	48.5 ± 2.1
BEFV+SU6656	19.6 ± 3.2	2.54 ± 0.82^*	83.1 ± 4.3^*	7.8 ± 1.7	2.36 ± 0.74^*	78.6 ± 5.7^*
BEFV+SP600125+SU6656	18.3 ± 2.8	2.86 ± 0.74^*	85.7 ± 3.9^*	7.1 ± 1.4	2.00 ± 0.81^*	82.7 ± 2.7^*

< 0.05 compared with BEFV-infected cells. The rate of apoptotic ELISA is shown as the enrichment factor. MTT assay results are expressed as a percentage normalized against mock cells.