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Table II.

Oligonucleotide primers used and plasmids constructed in this study.

Primer Sequences (5′ → 3′)a Plasmid
sopB P1(F) CAGAGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGCAAATACAGAGCTTC pTSopB
sopB P2(R) CCGAAGCTTTTACAGATCCTCTTCAGAGATGAGTTTCTGCTCAGATGTGATTAATGAAGA
sipB P1(F) CAGAGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGGTAAATGACGCAAGTAGCATTAG pTSipB
sipB P2(R) CCGCTGCAGTTACAGATCCTCTTCAGAGATGAGTTTCTGCTCTGCGCGACTCTGGCGCAGAATAAAACG
crp P1(F) CAGAGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGGTGCTTGGCAAACC pTCRP
crp P2(R) CCGAAGCTTTTACAGATCCTCTTCAGAGATGAGTTTCTGCTCACGGGTGCCGTAGACGA
crp P3(F) AATGCCGGATCCTGGACAGCAAGCGAACCGGAATTGCC p705CRP
crp P3(R) CGGGGATCCTCACACAGGAAACAGCTATGAC
a

The Myc-tag sequences are shown in italics. Restriction sites are underlined. Stop codons are shown in bold italics. Sequences of partial neomycin promoter of Tn5 transposon are shown in bold.