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Table I.
Primers used to clone pmp plasmid standards used in RT-PCR.
| Gene | Forward primer (5′-3′)* | Reverse primer (5′-3′)* | Amplicon size (bp) |
|---|---|---|---|
| pmp1B | GACGACGACAAGATCTCGATTACAGTATTTGGAGAAC | GAGGAGAAGCCCGGTTTAGAATATCATACGTGCGCCGCAGTTAG | 5351 |
| pmp2A | GACGACGACAAGATTTCACTCGCACCACTGACTC | GAGGAGAAGCCCGGTTTAGAAACTTAGAGATATACCACC | 2792 |
| pmp3E | GACGACGACAAGATTTTTACCTCTATTCTCGGAAGCC | GAGGAGAAGCCCGGTCTAAAAAATTAACGAGCTCCCTGC | 2937 |
| pmp4E | GACGACGACAAGATCGAACTCATTTGCCTTGCTAAG | GAGGAGAAGCCCGGTCTAAAATTGTAATTCACTTG | 2807 |
| pmp5E | ATAAGACCTGAGAAAACGATATGAAATCCAC | AGTTGCTAGAGAGTTTAGAAGGAAAGTTTGCCCCCAG | 1114 |
| pmp6H | GACGACGACAAGATTTGCTTATGCAGCTCGGGGTGG | GAGGAGAAGCCCGGTTTAGAATGTGCCTTGCAAGCCTGTGATG | 2930 |
| pmp7G | GACGACGACAAGATTAACGGCGACCTTAGAGAGATGTTTC | GAGGAGAAGCCCGGTTTAGAATCGAAATTTACTTCCTAC | 3002 |
| pmp10G | GACGACGACAAGATTGACGGCTCTACAGGGACC | GAGGAGAAGCCCGGTTTAACGAAGTTCGCCATTGCCTGA | 2408 |
| pmp11G | GACGACGACAAGATTCTGGCAACTGGATTCAACGGC | GAGGAGAAGCCCCGGTTTAAAAATGCACCCTGCTG | 2495 |
| pmp13G | GACGACGACAAGATCACGAACAGTGAGCCATTCAATC | GAGGAGAAGCCCGGTTTAGAAGCCAAATTGACTGAAGAGC | 2399 |
| pmp14G | GACGACGACAAGATCGACGCAAACGGAGCATTCAG | GAGGAGAAGCCCGGTTTAGAATGCGACCTTAGCG | 2699 |
| pmp15G | GACGACGACAAGATAAGCTCTAGCGATAATTACGATGG | GAGGAGAAGCCCGGTTTAGAATTTGTATCTACCC | 4110 |
| pmp16G | GACGACGACAAGATCTCCTATGCAGGCAAAGATTCTCC | GAGGAGAAGCCCGGTTTACAAGCCAAATTGACTGAAGAGC | 2321 |
| pmp17G | GACGACGACAAGATCTCCTTTGCAGGGAAAGATTCAGG | GAGGAGAAGCCCGGTTTAGGTTCGTGAAGATCCCCTGAGC | 2324 |
| pmp18D | GACGACGACAAGATGTCTACATTTTCTCACTCCG | GAGGAGAAGCCCGGTTTAGAAGATCAAGCGCATACCACAATTC | 4598 |
| omp1 | CATATGCCTGTAGGGAACCCAGCTGAACC | GGGCGAATTCTTATGCGAATGGAT | 1245 |
| 16S | GGCGGCGTGGATGAGGC | GAGTGTACTCCTCAGGCGGCA | 868 |