Western blot analyses of 16 experimentally haemoplasma infected cats and five SPF cats using a recombinant Mhf HspA1 as an antigen. (A) Western blot analysis of animals infected with different haemotropic Mycoplasmas (1: cat infected with Mhf; 2: cat infected with CMhm; 3: cat infected with CMt; 4–8: SPF cats). (B) Serum of groups A and B and the amplificatory cats collected prior to exposure. (C) Serum of groups A and B and the amplificatory cats collected post-exposure (5–11 weeks after inoculation with CMt-positive blood). M: Marker; 1–5: group A cats; 6–10: group B cats; 11–13: cats X, Y, and Z. Seroconversion occurred as early as three weeks p.i.; one cat seroconverted only at eight weeks after CMt inoculation. The arrow indicates the specific band (calculated size of 33.6 kDa).