Immunoblot detection of GlpO and GlpOΔFAD. (A) One-microgram samples of purified recombinant GlpO (lane 1) and GlpOΔFAD (lane 2) were run by SDS-10% polyacrylamide gels, transferred onto a nitrocellulose membrane and probed with anti-His antibody. (B) Total antigen (approximately 10 μg per lane) of M. mycoides subsp. mycoides SC strain Afadé was separated on SDS-10% polyacrylamide gels and immunoblotted using either anti-GlpO (lane 1) or anti-GlpOΔFAD antiserum (lane 2) at a dilution of 1:1000. Std, molecular mass standards.