Open Access
Table II.
Primers and probe sequences used in the four different allelic discrimination assays were designed using GenBank M31313.
| PCR mixture | Primer | MGB-probe | AA |
|---|---|---|---|
| Codon 136 | 136F: 5′-CTGCAGCTGGAGCAGTGGTA-3′ | 136Ala: 5′FAM-TCRTGgCACTTCC-3′ (300 nM) | Ala |
| 136R: 5′-GATAGTAACGGTCCTCATAGTCATTGC-3′ | 136Val: 5′VIC-CTCATGaCACTTCC-3′(200 nM) | Val | |
| Codon 154 | 154F: 5′TGGCAATGACTATGAGGACCG-3′ | 154Arg: 5′FAM-ACTATCgTGAAAACAT-3′(120 nM) | Arg |
| 154R: 5′-TGGTCTGTAGTACACTTGGTTGGG-3′ | 154His: 5′VIC-TACTATCaTGAAAACATG-3′(200 nM) | His | |
| Codon 171-1 | 171F: 5′-GTTACCCCAACCAAGTGTACTACAGA-3′ | 171Arg: 5′FAM-CCAGTGGATCgGTATA-3′(150 nM) | Arg |
| 171R: 5′-TGTTGACACAGTCATGCACAAAG-3′ | 171His: 5′-ACCAGTGGATCa TTAT-3′(120 nM) | His | |
| Codon 171-2 | 171F: 5′-GTTACCCCAACCAAGTGTACTACAGA-3′ | 171Arg: 5′FAM-CCAGTGGATCgGTATA-VIC (150 nM) | Arg |
| 171R: 5′-TGTTGACACAGTCATGCACAAAG-3′ | 171Gln: 5′VIC-ACCAGTGGATCaGTATA-3′ (200 nM) | Gln |