Open Access
Table I.
Primers and conditions used for qRT-PCR.
| Gene | Sequence | Ta°Ca | Total MgCl2b(in mM) |
|---|---|---|---|
| AGP | F: AGTCCTGAGCCTCCTTCCTC | 60 | 1.5 |
| R: GCCGAGCCGATATAATACCA | |||
| ALB | F: CAGAAGTTTTGGGGAAAATACCT | 60 | 3.0 |
| R: TAAAGGAGTTCTGGGGCGTA | |||
| APOA1 | F: GTTCTGGGACAACCTGGAAA | 60 | 3.0 |
| R: GCTGCACCTTCTTCTTCACC | |||
| B2M | F: TGAAGCACGTGACTCTCGAT | 62 | 1.5 |
| R: CTCTGTGATGCCGGTTAGTG | |||
| β-actin | F: CTACGTCGCCCTGGACTTC | 62 | 1.5 |
| R: GCAGCTCGTAGCTCTTCTCC | |||
| CRP | F: CTTTTGCCCAGACAGACATGAT | 60 | 1.5 |
| R: GAGTGGTTTGGTGAGCCTTG | |||
| FIB | F: GAATTTTGGCTGGGAAATGA | 59 | 3.0 |
| R: CAGTCCTCCAGCTGCACTCT | |||
| GAPDH | F: ACCCAGAAGACTGTGGATGG | 62 | 1.5 |
| R: AAGCAGGGATGATGTTCTGG | |||
| Hp | F: ACAGATGCCACAGATGACAGC | 60 | 1.5 |
| R: CGTGCGCAGTTTGTAGTAGG | |||
| HPRT1 | F: ACACTGGCAAAACAATGCAA | 62 | 1.5 |
| R: TGCAACCTTGACCATCTTTG | |||
| HTPAP | F: GCTCTTGGCAGCTGTGATTG | 59 | 3.0 |
| R: ATCCGTCAGGGGAGGGTAGT | |||
| PigMAP | F: ATGACAGCAAGCGAACAGTG | 60 | 1.5 |
| R: GGGGATCCCTCTTGGTAATC | |||
| RPL13A | F: ATTGTGGCCAAGCAGGTACT | 62 | 3.0 |
| R: AATTGCCAGAAATGTTGATGC | |||
| SAA2 | F: TAAAGTGATCAGCAATGCCAAA | 60 | 1.5 |
| R: TCAACCCTTGAGTCCTCCAC | |||
| TF | F: CTCAACCTCAAAACTCCTGGAA | 60 | 3.0 |
| R: CCGTCTCCATCAGGTGGTA | |||
| TTR | F: TTGCCTTGGGGAAAACCA | 60 | 1.5 |
| R: TGGTGTCCAATTCCACTTTG | |||
| IL-6 | F: TGGGTTCAATCAGGAGACCT | 60 | 3.0 |
| R: CAGCCTCGACATTTCCCTTA |