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Table II.
Histological observations of lesions from rabbits infected with wild-type or mutant viruses. All animals were subjected to a complete post-mortem examination. Tissue materials were from the injection site (ear [primary site]) and parotid lymph node (secondary site).
| Lesion intensitya with the indicated virus |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clinical signs | Day 5 p.i. |
Day 8 p.i. |
Day 13 p.i. |
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| Wild-type | MYXV-ΔM148R | MYXV-ΔM149R | MYXV-ΔM148R ΔM149R | Wild-type | MYXV-ΔM148R | MYXV-ΔM149R | MYXV-ΔM148R ΔM149R | Wild-type | MYXV-ΔM148R | MYXV-ΔM149R | MYXV-ΔM148R ΔM149R | |
| Primary siteb: Perivascular dermatitis | ||||||||||||
| Epidermal spongiosis and vesicles | – | – | – | – | ++++ | + | +++ | + | + | + | + | – |
| Edema | +++ | +++ | +++/++++ | ++/+++ | ++++ | +++ | ++ | ++ | +++ | ++ | +++ | ++ |
| Focal interstitial microhaemorrages | – | + | ++ | – | ++ | + | ++ | + | ++ | – | +++ | – |
| Focal thrombosis | + | + | – | + | ++ | + | ++ | + | – | – | ++ | + |
| Heterophils | ++/+++ | ++/+++ | ++/+++ | ++/+++ | ++++ | ++ | +++ | ++ | +++ | + | ++ | + |
| Mononuclear cells | + | + | + | + | ++ | ++++ | ++ | ++++ | +++ | ++++ | +++ | ++++ |
| Activated fibroblasts | ++ | ++ | ++ | ++ | +++ | +++ | +++ | +++ | +++++ | +++ | +++ | +++ |
| Interstitial mucinosis | +++ | +++ | +++ | ++ | +++ | ++ | +++ | +++ | ++++ | ++ | +++ | ++ |
| Secondary sitesc: Lymphadenitis | ||||||||||||
| Lymphodepletion | – | – | – | – | – | – | – | – | – | – | – | |
| Lymphoid hyperplasia | ++/+++ | ++ | ++ | ++ | +++ | +++ | +++ | ++ | ++ | ++ | ++ | |
| Focal histiocytosis | ++++ | +++ | +++/++++ | ++/+++ | +++ | +++ | +++ | +++ | ++ | +++ | +/– | |
| Infiltration by heterophils | +++ | +++ | +++ | ++ | +++ | ++ | +++ | + | – | + | – | |
| Activated fibroblasts | – | – | – | – | +++ | ++ | ++ | + | – | + | – | |
| Interstitial mucinosis | – | – | – | – | +++ | + | + | + | – | – | – | |