Issue |
Vet. Res.
Volume 40, Number 3, May-June 2009
|
|
---|---|---|
Number of page(s) | 15 | |
DOI | https://doi.org/10.1051/vetres:2008052 | |
Published online | 12 December 2008 | |
How to cite this article | Vet. Res. (2009) 40:14 |
DOI: 10.1051/vetres:2008052
Targeting to porcine sialoadhesin receptor receptor improves antigen presentation to T cells
Concepción Revilla, Teresa Poderoso, Paloma Martínez, Belén Álvarez, Laura López-Fuertes, Fernando Alonso, Angel Ezquerra and Javier DomínguezDpto. Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 28040, Madrid, Spain
Received 10 July 2008; accepted 10 December 2008; published online 12 December 2008
Abstract - Antibody-mediated targeting of antigen to specific
antigen presenting cells (APC) receptors is
an attractive strategy to enhance T cell immune responses to weak
immunogenic antigens. Here, we describe
the characterization of two monoclonal antibodies (mAb) against different
epitopes of porcine sialoadhesin
(Sn) and evaluate in vitro the potential of targeting this receptor for
delivery of antigens to APC for T cell
stimulation. The specificity of these mAb was determined by amino acid
sequence analysis of peptides
derived from the affinity purified antigen. Porcine Sn is expressed by
macrophages present in the border
between white and red pulp of the spleen and in the subcapsular sinus of
lymph nodes, an appropriate
location for trapping blood and lymph-borne antigens. It is also expressed
by alveolar macrophages
and monocyte-derived dendritic cells (MoDC). Blood monocytes are negative
for this molecule, but its
expression can be induced by treatment with IFN-. MAb bound to Sn is
rapidly endocytosed. MAb to
sialoadhesin induced in vitro T cell proliferation at concentrations
100-fold lower than the non-targeting
control mAb when using T lymphocytes from pigs immunized with mouse
immunoglobulins as responder
cells and IFN-
treated monocytes or MoDC as APC, suggesting a role of
sialoadhesin in antigen uptake and/or delivery into the presentation pathway in APC.
Key words: sialoadhesin / monoclonal antibody / macrophage / antigen-targeting / swine
Corresponding author: crevilla@inia.es
© INRA, EDP Sciences 2009