| Issue |
Vet. Res.
Volume 38, Number 6, November-December 2007
|
|
|---|---|---|
| Page(s) | 871 - 882 | |
| DOI | https://doi.org/10.1051/vetres:2007035 | |
| Published online | 20 September 2007 | |
| How to cite this article | Vet. Res. (2007) 871-882 | |
DOI: 10.1051/vetres:2007035
Morphometric analysis of proinflammatory cytokines in mammary glands of sows suggests an association between clinical mastitis and local production of IL-1beta, IL-6 and TNF-alpha
Yaohong Zhua, b, Caroline Fossumc, Mikael Bergd and Ulf Magnussona, ba Division of Reproduction, Department of Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, PO Box 7054, 75007 Uppsala, Sweden
b Centre for Reproductive Biology in Uppsala, Uppsala, Sweden
c Division of Immunology, Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, 75123 Uppsala, Sweden
d Division of Parasitology and Virology, Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, 75007 Uppsala, Sweden
(Received 13 February 2007; accepted 11 June 2007; published online 20 September 2007)
Abstract - Twelve healthy primiparous sows received
intramammary inoculation with Escherichia coli (serotype O127) during the 24-h period
preceding parturition. Mammary gland biopsy samples were taken immediately
before inoculation (0 h) and from the inoculated and the contralateral
non-inoculated glands 24 h after inoculation. The analyses of interleukin-1
beta (IL-1
), IL-6, IL-8, and tumor necrosis factor alpha
(TNF-
) by immunohistochemistry revealed that the production of
these proinflammatory cytokines significantly increased in the inoculated
mammary glands of sows that developed clinical signs of mastitis (affected
group, n=4) 24 h after inoculation. This was also true for IL-8 in the
inoculated mammary glands of sows that did not develop clinical signs of
mastitis (nonaffected group, n=8). Sows that developed clinical signs of
mastitis displayed significantly lower constitutive production of IL-1 than did sows that remained clinically healthy. The data indicate that the
development of clinical symptoms of coliform mastitis in the sow is
associated with a locally increased proinflammatory cytokine production in
response to intramammary E. coli infection.
Key words: cytokine / immunohistochemistry / mastitis / pig / E. coli
Corresponding author: ulf.magnusson@kv.slu.se
© INRA, EDP Sciences 2007