Free Access
Vet. Res.
Volume 38, Number 3, May-June 2007
Page(s) 375 - 390
Published online 13 March 2007
How to cite this article Vet. Res. (2007) 375-390
Vet. Res. 38 (2007) 375-390
DOI: 10.1051/vetres:2007001

Vesicular stomatitis New Jersey virus (VSNJV) infects keratinocytes and is restricted to lesion sites and local lymph nodes in the bovine, a natural host

Charles F.C. Scherera, b, c, Vivian O'Donnella, d, William T. Goldea, Douglas Gregga, D. Mark Estesb, c and Luis L. Rodrigueza

a  Plum Island Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, PO Box 848, Greenport, NY 11944, USA
b  University of Missouri, College of Veterinary Medicine, Department of Veterinary Pathobiology, Columbia, MO 65251, USA
c  Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA
d  Department of Pathobiology, University of Connecticut, Storrs CT 06269, USA

(Received 16 August 2006; accepted 19 October 2006 ; published online 13 March 2007)

Abstract - Inoculation of vesicular stomatitis New Jersey virus (VSNJV) by skin scarification of the coronary-band in cattle, a natural host of VSNJV, resulted in vesicular lesions and 6-8 log10 TCID50 increase in skin virus titers over a 72 h period. Virus infection was restricted to the lesion sites and lymph nodes draining those areas but no virus or viral RNA was found in the blood or in 20 other organs and tissues sampled at necropsy. Scarification of flank skin did not result in lesions or a significant increase in viral titer indicating that viral clinical infection is restricted to skin inoculation at sites where lesions naturally occur. Viral antigens co-localized primarily with keratinocytes in the coronary band, suggesting these cells are the primary site of viral replication. Viral antigen also co-localized with few MHC-II positive cells, but no co-localization was observed in cells positive for macrophage markers. Although granulocyte infiltration was observed in lesions, little viral antigen co-localized with these cells. This is the first detailed description of VSNJV tissue distribution and infected cell characterization in a natural host. The pathogenesis model shown herein could be useful for in-vivo tracking of virus infection and local immune responses.

Key words: vesicular stomatitis / bovine / pathogenesis / confocal microscopy / keratinocytes

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© INRA, EDP Sciences 2007