Open Access
Vet. Res.
Volume 37, Number 5, September-October 2006
Page(s) 705 - 723
Published online 06 July 2006
How to cite this article Vet. Res. (2006) 705-723
Vet. Res. 37 (2006) 705-723
DOI: 10.1051/vetres:2006027

Cellular gene expression survey of PseudoRabies Virus (PRV) infected Human Embryonic Kidney cells (HEK-293)

Yannick Blancharda, b, Nolwenn Le Meurc, Martine Le Cunffc, Philippe Blancharda, Jean Légerc and André Jestina

a  Laboratoire de Génétique Virale et Biosécurité, Agence Française de Sécurité Sanitaire des Aliments, BP 53, 22440 Ploufragan, France
b  Zoopôle Développement, 7 rue Jean Rostand, 22440 Ploufragan, France
c  INSERM, U 533, Hôpital Hôtel-Dieu, BP 1005, 44093 Nantes, France

(Received 23 September 2005; accepted 21 February 2006; published online 6 July 2006)

Abstract - Pseudorabies virus (PRV) is an alpha herpesvirus that causes Aujezsky disease in the pig. To characterize the impact of PRV infection on cellular expression, we used microarrays consisting of 9850 oligonucleotides corresponding to human genes and examined the expression levels of mRNA isolated 0.5, 3, 6, and 9 h post infection (hpi) from cultures of infected HEK-293 cells. Very few changes were observed during the first 3 h of infection but significant modifications in the cell expression of more than 1000 genes were clearly apparent by 6 hpi. More than 2400 genes were either up- or down-regulated during the 9 h experiment. These results were then analyzed using gene ontology and the MAPP and MAPPFinder software. This comprehensive analysis clearly shows that the down-regulated genes were mainly involved in macromolecular synthesis (DNA, RNA and proteins) and the cell cycle. The up-regulated genes primarily concerned the regulation of DNA transcription, developmental processes (central nervous system development, neurogenesis, angiogenesis), cell adhesion and potassium transport. This study is the first qualitative analysis of a gene expression survey in a human cell line following PRV infection. It demonstrates global changes in the cell expression profile, and identifies the main biological processes that are altered during virus replication.

Key words: pseudorabies virus / microarray / host pathogen interaction

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© INRA, EDP Sciences 2006