Free Access
Vet. Res.
Volume 37, Number 4, July-August 2006
Page(s) 553 - 564
Published online 28 April 2006
How to cite this article Vet. Res. (2006) 553-564
Vet. Res. 37 (2006) 553-564
DOI: 10.1051/vetres:2006018

Sensitivity and specificity of the agar-gel-immunodiffusion test, ELISA and the skin test for detection of paratuberculosis in United States Midwest sheep populations

Suelee Robbe-Austermana, Ian A. Gardnerb, Bruce V. Thomsenc, Daniel G. Morricald, Barbara M. Martinc, Mitchell V. Palmera, Charles O. Thoene and Chad Ewingf

a  National Animal Disease Center, Agricultural Research Service, USDA, 2300 Dayton, Ames, Iowa 50010, USA
b  Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA
c  National Veterinary Services Laboratories, USDA, 1800 Dayton, Ames, Iowa 50010, USA
d  Department of Animal Sciences, Iowa State University, Ames, Iowa 50011, USA
e  Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, IA 50011, USA
f  Groton Veterinary Clinic, 1503 N Broadway, Groton, SD 57445, USA

(Received 24 May 2005; accepted 13 January 2006; published online 28 April 2006)

Abstract - Our objective was to estimate the sensitivity and specificity of the agar-gel-immunodiffusion test (AGID), the ELISA, and the skin test for the detection of Mycobacterium avium subspecies paratuberculosis (MAP) in sheep using Bayesian methods without a gold standard. Fourteen flocks (2 465 sheep) were used. Five flocks (450 sheep) were considered MAP non-infected and 9 flocks (2 015 sheep) had sheep infected with MAP. Sheep were skin tested and blood was collected for AGID and ELISA testing. Results were analyzed using a Bayesian 3-test in 1-population model fitted in WinBUGS. The model allowed for dependence (correlation) between the two serologic tests, but these two tests were assumed to be conditionally independent of the skin test. The estimated specificity was 99.5% (95% PI of 98.9-99.9%) for the AGID; 99.3% (98.4-99.8%) for the ELISA using an optical density measured cutoff of 0.20; 99.2% (98.1-99.8%) using a cutoff of 0.15; 97.5% (95.8-98.7%) using a cutoff of 0.10; and 98.7% (97.3-99.5%) for the skin test. The estimated sensitivities were 8.3% (6.2-10.7%) for the AGID; 8.0% (6.0-10.4%), 10.6% (8.3-13.1%), and 16.3% (13.5-19.4%) for the ELISA using the cutoffs 0.20, 0.15, and 0.10 respectively; and 73.3% (62.3-85.8%) for the skin test. The skin test was specific in non-infected populations and sensitive in infected populations, although in some cases a positive skin test might represent MAP exposure rather than infection. The AGID and ELISA were specific but lacked sensitivity. The AGID and ELISA consistently identified two different populations of infected sheep with only moderate overlap between positive test results.

Key words: Johne's disease / sheep / ELISA / AGID / skin test

Corresponding author: Suelee Robbe-Austerman

© INRA, EDP Sciences 2006