Serum amyloid A and TNF in serum and milk during experimental endotoxin mastitisTanja Lehtolainena, Christine Røntvedb and Satu Pyöräläa
a Department of Clinical Veterinary Science, Faculty of Veterinary Medicine, University of Helsinki, 04920 Saarentaus, Finland
b Danish Institute of Agricultural Sciences, Research Centre Foulum, Department of Animal Health and Welfare, PO Box 50, 8830 Tjele, Denmark
(Received 17 December 2003; accepted 13 May 2004)
Abstract - A cross-over study was conducted to investigate the effect of intramammarily infused lipopolysaccharide (LPS) on the acute phase reaction in early (EL) and in late (LL) lactation. Nine cows received intramammary injections of 100 µg of Escherichia coli 0111:B4 LPS during EL and LL. The severity of each cows systemic and local signs and change in milk appearance were recorded and scored throughout the experiment. Systemic and local signs were found to be more serious in EL cows. Tumor necrosis factor (TNF ) was detected in milk but not in serum. Serum amyloid A (SAA) concentrations increased both in serum and in milk. The milk TNF concentrations peaked at 8 h post-challenge (PC). SAA concentrations started to increase at 8 h PC, and peak concentrations were seen at 32 and 48 h PC in milk and serum, respectively. The milk TNF and SAA seemed to be correlated, being on average higher in EL. Serum SAA concentration was not correlated with milk TNF or SAA, nor with the severity of local or systemic signs, but was correlated with changes in milk appearance.
Key words: LPS / mastitis / dairy cow / SAA / TNF
Corresponding author: Tanja Lehtolainen email@example.com
© INRA, EDP Sciences 2004