Characterization of a novel activation antigen on porcine lymphocytes recognized by monoclonal antibody 5A6/8Nieves Doménecha, Rosario Bullidob, Belén Álvarezb, Emilia Camposb, María-Pilar Rodríguez-Carreñob, Manuel Gómez del Moralb, Fernando Alonsob, Ángel Ezquerrab and Javier Domínguezb
a Laboratory of Investigation, Juan Canalejo Medical Center, Xubias de Arriba, 84, 15006 La Coruña, Spain
b Department of Biotechnology, INIA, 28040 Madrid, Spain
(Received 22 December 2003; accepted 19 February 2004)
Abstract - In this report, we describe the characterization of a novel activation antigen on porcine lymphocytes recognized by mAb 5A6/8. This antigen was detected on B and T cells 24 h after treatment with various stimuli. It was also found on alveolar macrophages, and at low levels on untreated monocytes. MAb 5A6/8 precipitated two bands of 45 and 50 kDa under non-reducing conditions, and of 22 and 28 kDa under reducing conditions. The cellular distribution, expression kinetics and/or molecular size of the 5A6/8 antigen differ from those of other known lymphocyte activation antigens. MAb 5A6/8 was able to inhibit lymphocyte proliferative responses driven by different stimuli, suggesting a role for this molecule in the events that lead to lymphocyte activation.
Key words: activation antigen / lymphocyte / monoclonal antibody / monocyte / pig
Corresponding author: Nieves Doménech firstname.lastname@example.org
© INRA, EDP Sciences 2004