Free access
Vet. Res.
Volume 35, Number 3, May-June 2004
Page(s) 275 - 282
How to cite this article Vet. Res. (2004) 275-282
Vet. Res. 35 (2004) 275-282
DOI: 10.1051/vetres:2004009

Haptoglobin and pig-major acute protein are increased in pigs with postweaning multisystemic wasting syndrome (PMWS)

Joaquim Segalésa, Carlos Piñeirob, Fermín Lampreavec, Miquel Nofraríasa, Enric Mateua, Maria Calsamigliaa, Marta Andrésc, Joaquín Moralesb, Matilde Piñeiroc and Mariano Domingoa

a  Centre de Recerca en Sanitat Animal (CReSA), Departament de Sanitat i d'Anatomia Animals, Facultat de Veterinaria, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain
b  PigCHAMP Pro Europa, S.A., Grupo Proinserga, Segovia, Spain
c  Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, Zaragoza, Spain

(Received 15 October 2003; accepted 8 December 2003)

Abstract - The objective of this study was to determine the serum concentration levels of selected acute phase proteins (APP), haptoglobin (HPT) and pig-major acute phase protein (pig-MAP), in postweaning multisystemic wasting syndrome (PMWS) affected pigs and PCV2-subclinically infected pigs. In a first study, a group of 15 eight-week-old conventional pigs from a PMWS affected farm were bled and a complete necropsy, histopathology and in situ hybridisation to detect PCV2 were performed. Based on the results, pigs were classified as suffering from PMWS (n = 10) or healthy animals (n = 5). In a second study, a group of 45 pigs from another PMWS affected farm were selected and bled at 3, 7, 12 and 28 weeks of age. The assessment of PCV2 infection status in these pigs was retrospectively done by PCV2 PCR in serum samples. Selected APP were measured in the serum of all studied pigs by means of radial immunodiffusion. Mean HPT and pig-MAP levels were significantly increased (p = 0.004 and p = 0.0006 respectively) in PMWS-affected pigs when compared to levels found in healthy pigs (2.52 ± 0.88 mg/mL vs. 1.06 ± 0.73 mg/mL for HPT and 3.81 ± 1.53 mg/mL vs. 0.76 ± 0.34 mg/mL for pig-MAP). In the second study, no significant difference in mean HPT and pig-MAP values were observed between PCV2 PCR positive and negative pigs of any age. However, both APP increased significantly with age in PCV2 PCR negative pigs. Altogether, the present results suggest that APP levels are significantly increased in pigs that develop PMWS, but not in animals with a PCV2 subclinical infection.

Key words: porcine circovirus type 2 (PCV2) / postweaning multisystemic wasting syndrome (PMWS) / acute phase proteins / haptoglobin / pig major acute phase protein (pig-MAP)

Corresponding author: Joaquim Segalés

© INRA, EDP Sciences 2004