Open Access
Table I.
Primers used to amplify the vtaA passenger domains or fragments of the vtaA10 passenger domain. The size of the amplified fragment (bp) is indicated in the first column. All primers contained a restriction enzyme BsaI target except for vtaA1 which contained a restriction enzyme BsmBI target (underlined and in bold).
| Gene | Primer name | Sequence |
|---|---|---|
| vtaA Haemophilus parasuis strain Nagasaki | ||
| vtaA1 (6 792 bp) | adh7-bsm-L2(-)F | ATGGTA CGTCTCA AATGGCTGCATATCTTCAAGATGGTGC |
| adh7-bsm-3′less | ATGGTA CGTCTCA GCGCTAACAATATCTCTTGATAATTGATTAAATC | |
| vtaA2 (3 221 bp) | adh3-L2(-)F | ATGGTA GGTCTCA AATGGCTGCATATTTTCAAGATAGTTCTG |
| G1-3′less | ATGGTA GGTCTCA GCGCTACGACCAATATCTCTTGATAATTGAT | |
| vtaA3 (3 138 bp) | adh15-L2(-)F | ATGGTA GGTCTCA AATGGCAGCATGGTTGGTAGATGGTTC |
| adh15-y(-)R | ATGGTA GGTCTCA GCGCTAACAATATCTCTTGATAATTGATTAAATC | |
| vtaA4 (3 095 bp) | adh23-L2(-)F | ATGGTA GGTCTCA AATGTCAGCTGCATATTTTGCAGGTGG |
| G1-3′less | ATGGTA GGTCTCA GCGCTACGACCAATATCTCTTGATAATTGAT | |
| vtaA5 (2 720 bp) | adh1/21-L2(-)F | ATGGTA GGTCTCA AATGGGTGCACAACTTCACACAGGAAC |
| G1-3′less | ATGGTA GGTCTCA GCGCTACGACCAATATCTCTTGATAATTGAT | |
| vtaA6 (2 828 bp) | adh19-L2(-)F | ATGGTA GGTCTCA AATGTCAGCTACTCACTCAGGAACACC |
| G1-3′less | ATGGTA GGTCTCA GCGCTACGACCAATATCTCTTGATAATTGAT | |
| vtaA7 (2 618 bp) | adh25-L2(-)F | ATGGTA GGTCTCA AATGGCAGATCGTCATAAAGGAACTCC |
| G1-3′less | ATGGTA GGTCTCA GCGCTACGACCAATATCTCTTGATAATTGAT | |
| vtaA8 (2 756 bp) | adh9-L2(-)F | ATGGTA GGTCTCA AATGGCAAGGCTTTTTCTAGATGAGGC |
| G1-3′less | ATGGTA GGTCTCA GCGCTACGACCAATATCTCTTGATAATTGAT | |
| vtaA9 (2 405 bp) | adh1/21-L2(-)F | ATGGTA GGTCTCA AATGGGTGCACAACTTCACACAGGAAC |
| G1-3′less | ATGGTA GGTCTCA GCGCTACGACCAATATCTCTTGATAATTGAT | |
| vtaA10 (4 488 bp) | adh11-L2(-)F | ATGGTA GGTCTCA AATGGCATATCTTGAATATGGTGCTCGA |
| adh5-3′less | ATGGTA GGTCTCA GCGCTACGGTTGATCTTATTGTGAATATCG | |
| vtaA11 (4 047 bp) | adh5-L2(-)F | ATGGTA GGTCTCA AATGTCAATTACTGATTCATCATATTTTCAAC |
| adh5-3′less | ATGGTA GGTCTCA GCGCTACGGTTGATCTTATTGTGAATATCG | |
| vtaA12 (4 013 bp) | adh17-L2(-)F | ATGGTA GGTCTCA AATGGCAAGGCTTTTTCTAGATGGTGC |
| G3-3′less | ATGGTA GGTCTCA GCGCTTCTCTCTAACTTCGCACCCAATC | |
| vtaA13 (3 752 bp) | adh13-L2(-)F | ATGGTA GGTCTCA AATGGCTAACACAACAAATACCGCAAAAC |
| G3-3′less | ATGGTA GGTCTCA GCGCTTCTCTCTAACTTCGCACCCAATC | |
| vtaA10 Haemophilus parasuis strain Nagasaki partial constructions | ||
| vtaA10 5′ (1 562 bp) | adh11-L2(-)F | ATGGTA GGTCTCA AATGGCATATCTTGAATATGGTGCTCGA |
| adh11parcial_5′R | ATGGTA GGTCTCA GCGCTCATAGCGGCAATCGTATCAATTTTA | |
| vtaA10 central (1 004 bp) | adh11parcial_CF | ATGGTA GGTCTCA AATGGATAAAATTGATACGATTGCCGCTA |
| adh11parcial_CR | ATGGTA GGTCTCA GCGCTTTTATCCGTTTGATTTTCGTCTTTCG | |
| vtaA10 3′ (1 980 bp) | adh11parcial_3′F | ATGGTA GGTCTCA AATGGTTGGTCCGAAAGACGAAAATCAA |
| adh11parcial_3′R | ATGGTA GGTCTCA GCGCTACGGTTGATCTTATTGTGAATATCG | |
| vtaA Haemophilus parasuis strain HP1319 | ||
| vtaA15 (2 828 bp) | adh19-L2(-)F | ATGGTA GGTCTCA AATGTCAGCTACTCACTCAGGAACACC |
| G1-3′less | ATGGTA GGTCTCA GCGCTACGACCAATATCTCTTGATAATTGAT | |
| vtaA16 (2 765 bp) | adh9HP1319L2(-)F | ATGGTA GGTCTCA AATGTCAGCTGCTGCTAACACAACAAAT |
| G1-3′less | ATGGTA GGTCTCA GCGCTACGACCAATATCTCTTGATAATTGAT | |