Open Access
Table II.
Primer list. Primers used for constructs for expression in E. coli. Restriction sites are underlined. Bold indicates overlapping regions for splicing by overlap extension.
| Primer name | Primer sequence (5′-3′) | Construct | Primers used |
|---|---|---|---|
| GtxUP-NcoI | AGTCCCATGGGTCTTTCATTAAAAGAAAAAGTAACTGGAATA | gtxA+C | GtxUP-NcoI & gtxC-r-XhoI |
| gtxC-r-XhoI | CAGTCTCGAGTTATGAATTTTCTTCTATAAAAGCAGC | gtxA | GtxUP-NcoI & gtxA-r-XhoI |
| gtxA Cf NcoI | AGTCCCATGGCAATTGAATCTTTCAATTTAATCGCAA | RTX+C | gtxA Cf NcoI & gtxC-r-XhoI |
| gtxA-Nr-XhoI | CAGTCTCGAGTTAATTTAGGAAATCGGTCATTATGCCAT | RTX | gtxA Cf NcoI & gtxA-r-XhoI |
| gtxA-r-XhoI | CAGTCTCGAGTTAAACAAGATACATAGTGACCAGTTCAT | Nterm+C | GtxUP-NcoI & SOErev1 |
| SOErev1 | GTTATCCATAATAATTAATTTAGGAAATCGGTCATTATG | SOEfor2 & gtxC-r-XhoI | |
| SOEfor2 | TTCCTAAATTAATTATGGATAACTTCTCAACTTTAGG | Nterm | GtxUP-NcoI & gtxA-Nr-XhoI |