Western blot of NetB toxin from C. perfringens isolates from diseased chickens. Proteins were separated by 4–12% SDS-PAGE. SeeBlue Plus2 prestained marker (Invitrogen) was used as a size marker. Bands were transferred onto PVDF membranes and probed with rabbit polyclonal anti-rNetB antibody. Blots were developed with an ECL Western blotting kit and the results recorded on autoradiographic film. Strains: (1) EHE-NE18; (2) NAG-NE1; (3) 48; (4) 56; (5) 301001-1-B1.