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Table I.
Sequence analysis of clones up-regulated by fed mites. Each sequence was BLAST searched under NCBI and TIGR databases. The E value is a statistical value of the similarity of the two sequences being compared calculated by the BLAST programme [1]. The length of the alignment describes the length (in nucleotides) of the region of similarity between the two sequences.
Classification | N | Function | Homologue | E value | Identity (%) | Similarity (%) | Length of alignment |
---|---|---|---|---|---|---|---|
Allergens | 12 | Pso o I allergen | Psoroptes ovis | 1e-22 | 100 | 100 | 233 |
Group 5 allergen | Dermatophagoides pteronyssinus | 5e-18 | 61 | 77 | 74 | ||
Group 7 allergen | Dermatophagoides pteronyssinus | 9e-32 | 61 | 82 | 74 | ||
Group 13 allergen | Suidasia medanensis | 9e-04 | 47 | 68 | 47 | ||
Secreted proteins | 5 | Salivary gland protein | Anopheles gambiae | 3e-31 | 92 | 98 | 203 |
Oesophageal gland protein | Heterodera glycines | 6e-70 | 93 | 93 | 122 | ||
Salivary gland protein | Ixodes scapularis | 0.012 | 63 | 66 | 35 | ||
Antioxidant enzymes | 1 | Superoxide dismutase | Taiwanofungus camphorates | 0.004 | 91 | 91 | 24 |
Carbohydrate metabolism | 2 | Phosphoglycerate kinase | Schistosoma mansoni | 8e-29 | 57 | 74 | 107 |
Enolase | Glycyphagus domesticus | 6e-45 | 77 | 88 | 131 | ||
Lipid metabolism | 2 | Phosphatidylethanolamine binding protein | Tribolium castaneum | 1e-38 | 66 | 80 | 109 |
Fatty acid binding protein | Suidasia medanensis | 9e-04 | 47 | 68 | 47 | ||
Cell processes | 7 | ATP synthase | Rhipicephalus sanguineus | 9e-22 | 56 | 81 | 47 |
Methyl transferase | Drosophila pseudo-obscura | 4e-11 | 41 | 61 | 116 | ||
Cytochrome c oxidase | Archichauliodes sp. | 3e-16 | 45 | 62 | 82 | ||
Cytochrome monooxygenase | Zea mays | 2e-32 | 45 | 54 | 220 | ||
Cadherin 1 | Gallus gallus | 8e-05 | 44 | 61 | 58 | ||
Cadherin-like protein | Rhipicephalus appendiculatus | 4e-50 | 81 | 81 | 128 | ||
Ribosomal RNA | Ixodes ricinus | 4e-65 | 82 | 82 | 159 | ||
Other | 4 | Alpha haemolysin homologue | Ixodes scapularis | 1e-40 | 76 | 76 | 213 |
Serum opacity-like factor | Ixodes scapularis | 2e-20 | 79 | 79 | 100 | ||
Cell adhesion molecule | Tribolium castaneum | 7e-15 | 45 | 65 | 218 | ||
Cuticle protein DFP1 | Dermatophagoides pteronyssinus | 2e-11 | 68 | 72 | 86 |