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Table I.

Sequence analysis of clones up-regulated by fed mites. Each sequence was BLAST searched under NCBI and TIGR databases. The E value is a statistical value of the similarity of the two sequences being compared calculated by the BLAST programme [1]. The length of the alignment describes the length (in nucleotides) of the region of similarity between the two sequences.

Classification N Function Homologue E value Identity (%) Similarity (%) Length of alignment
Allergens 12 Pso o I allergen Psoroptes ovis 1e-22 100 100 233
Group 5 allergen Dermatophagoides pteronyssinus 5e-18 61 77 74
Group 7 allergen Dermatophagoides pteronyssinus 9e-32 61 82 74
Group 13 allergen Suidasia medanensis 9e-04 47 68 47
Secreted proteins 5 Salivary gland protein Anopheles gambiae 3e-31 92 98 203
Oesophageal gland protein Heterodera glycines 6e-70 93 93 122
Salivary gland protein Ixodes scapularis 0.012 63 66 35
Antioxidant enzymes 1 Superoxide dismutase Taiwanofungus camphorates 0.004 91 91 24
Carbohydrate metabolism 2 Phosphoglycerate kinase Schistosoma mansoni 8e-29 57 74 107
Enolase Glycyphagus domesticus 6e-45 77 88 131
Lipid metabolism 2 Phosphatidylethanolamine binding protein Tribolium castaneum 1e-38 66 80 109
Fatty acid binding protein Suidasia medanensis 9e-04 47 68 47
Cell processes 7 ATP synthase Rhipicephalus sanguineus 9e-22 56 81 47
Methyl transferase Drosophila pseudo-obscura 4e-11 41 61 116
Cytochrome c oxidase Archichauliodes sp. 3e-16 45 62 82
Cytochrome monooxygenase Zea mays 2e-32 45 54 220
Cadherin 1 Gallus gallus 8e-05 44 61 58
Cadherin-like protein Rhipicephalus appendiculatus 4e-50 81 81 128
Ribosomal RNA Ixodes ricinus 4e-65 82 82 159
Other 4 Alpha haemolysin homologue Ixodes scapularis 1e-40 76 76 213
Serum opacity-like factor Ixodes scapularis 2e-20 79 79 100
Cell adhesion molecule Tribolium castaneum 7e-15 45 65 218
Cuticle protein DFP1 Dermatophagoides pteronyssinus 2e-11 68 72 86