Open Access

Figure 4.


BEFV infection induced JNK phosphorylation and JNK activity. (A) Vero cells infected with 2 moi BEFV and (B) MDBK cells infected with 5 moi BEFV were cultured for the indicated additional length of time, and JNK phosphorylation was detected using specific phosphorylated JUN (p-JNK) antibody. (C) JNK kinase activity assay. Cell lysates collected from Vero cells (upper) infected with 2 moi and MDBK cells (lower) infected with BEFV for 48 h were subjected to the JNK kinase assay using c-Jun as the substrate. SP600125 specifically reversed the JNK activity signals.

Download original image