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Table I.
Primers.
| Primers sequence 5′ → 3′ | |
|---|---|
| PCR target/Genomic PTX3 exon2 | |
| Forward | ACACACTGTTTTTCGGAGGTGTC |
| Reverse | CGAGACATCCCAGAAGATAGCC |
| RT-PCR target/PTX3 cDNA | |
| Forward | GACAACGAAATAGACAATGGACTC |
| Reverse | CTTTGTGCCATAGGAAAACAGGAT |
| Forward | TCTGTGGCTTTGACCCAAATGC |
| Reverse | GAAATAGACAATGGACTTCATCC |
| RT-qPCR target/PTX3 | |
| Forward | TGGCCAAAACAAAAATAACCAG |
| Reverse | GTGCTTTCAGATGAATGCTGT |
| RT-qPCR housekeeping gene/GAPDH | |
| Forward | CCTTCTCTTGCTGGGTGATTG |
| Reverse | GACAATGAATTTGGCTACAGCA |