PTX3 specific antibody. (A) Using MNB1 human monoclonal primary antibody (which detects the C-terminal extremity of the PTX3 protein in humans), Western blotting revealed distinct masses for PTX3 protein corresponding to 32 and 42 kDa. (B) No band was detected using an IgG2b isotype control. The Western blotting was obtained using BALF cell culture supernatant. The cells originated from R.A.O.-affected horses in remission (named SN1 and SN2) and were challenged for 6 h with dust suspension (10 μg/mL).