Perforin content and morphology of ovine CD16⁺/CD14⁻ cells; comparison with bovine NKp46⁺ cells. (A) PBMC freshly obtained from sheep were triple labelled with the anti-CD16, anti-CD14 and anti-perforin mAbs: five different subsets (R0 to R4) were analyzed for perforin expression according to CD16 and CD14 expression (solid line, empty histogram). Shaded histograms represent labelling with the isotype control. Data shown are from 1 animal representative of 4. (B, top) Phase-contrast microscopy and (B, bottom) May–Grünwald–Giemsa staining of expanded ovine CD16⁺/CD14⁻ and bovine NKp46⁺ cells. Pictures shown are from 1 culture representative of 6 for ovine cells, and more than 10 for bovine cells. (C) Perforin labelling of expanded ovine CD16⁺/CD14⁻ and bovine NKp46⁺ cells counterstained with Evans blue. The cells shown are from 1 culture representative of 4 for both species.