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Figure 5.


Infection of ATE cells with IBV is not significantly affected by GAG. The effects of GAG on JEV and IBV infections were measured in BHK-21 cells (A) and ATE cells (B), respectively. The cells were seeded at a density of 5 × 103 cells/well in 96-well plates. The GAG were serially diluted and applied to BHK-21 cells and ATE cells prior to infection with 500 PFU of JEV and 103 EID50 of IBV at 37 °C for 1 h. The infected cells were fixed at 8 h.p.i. The number of infected cells was manually counted after immunocytostaining for IBV antigens. The data represent the average of triplicate wells under the same experimental conditions.

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