Cell identification and cell percentages of the primary ATE cells. The cell types of the cultured cells were identified by immunocytostaining. β-tubulin IV, ciliated cells; mucin 5AC, goblet cells; E-cadherin and ZO-1, epithelial cells; K14 and GSI-B4, basal cells; smooth muscle actin (SMA), smooth muscle cells; vimentin, fibroblast cells. The percentages of individual cell types among the ATE cells are illustrated. The data represent triplicate experiments that analyzed at least 500 counted cells. Scar bar, 25 μm. (A color version of this figure is available at www.vetres.org.)